Learn with ASCP : ASCP-MLT training material for 100% pass

Last Updated: Sep 07, 2026

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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Urinalysis and Body Fluids- Fluid analysis and interpretation
  • 1. CSF and other body fluids
    • 2. Urine chemical and microscopic examination
      Laboratory Operations- Quality and safety in the clinical lab
      • 1. Quality control and assurance
        • 2. Laboratory safety and regulations
          Clinical Chemistry- Metabolic and analytical testing
          • 1. Endocrine and metabolic panels
            • 2. Enzymes and electrolytes
              Microbiology- Clinical microbiological techniques
              • 1. Bacteriology and organism identification
                • 2. Culture and sensitivity testing
                  Hematology- Blood cell analysis and disorders
                  • 1. CBC and differential interpretation
                    • 2. Anemias and leukemias overview
                      Blood Banking / Immunohematology- Blood group systems and antibody identification
                      • 1. Crossmatching procedures
                        • 2. ABO and Rh systems

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          Question 1

                          The DAT, or direct antiglobulin test, is used to detect antibodies coating RBC's in vivo.
                          The Direct Antiglobulin Test (DAT) is usually used to detect:

                          A. Antibody-hapten reactions
                          B. Antigens in the plasma
                          C. Antibodies in the plasma
                          D. Antigens coating red cells
                          E. Antibodies coating red cells


                          Question 2

                          Entamoeba gingivalis resembles Entamoeba histolytica both in size and in nuclear characteristics. Entamoeba gingivalis may contain numerous cytoplasmic inclusions such as red blood cells, white blood cells, and bacteria.

                          I reside in the mouth where I measure approximately 17 micro meters.

                          A. Acanthamoeba species trophozoite
                          B. Entamoeba gingivalis trophozoite
                          C. Entamoeba coli trophozoite
                          D. Trichomonas vaginalis trophozoite


                          Question 3

                          Which of the following legislation was developed to protect the privacy of all patient information in the health care?

                          A. Health Insurance Portability and Accountability Act (HIPPA)
                          B. Occupational Safety and Health Administration (OSHA)
                          C. Clinical Laboratory Improvement Amendments (CLIA)
                          D. Clinical Laboratory Standards Institute (CLSI)


                          Question 4

                          Unconjugated bilirubin is a byproduct of RBC breakdown, or hemolysis. It would be expected to see an increase in unconjugated bilirubin when hemolysis is occuring at an increased rate. The liver enzymes would not remain at normal levels if there were a viral infection of the liver, chemical damage to the liver, or obstruction of bile ducts.
                          Chemistry
                          A patient presents with an elevation of unconjugated bilirubin, normal serum alkaline phosphatase, normal liver enzymes, and no bilirubin in the urine. This combination would suggest:

                          A. Viral infection of the liver.
                          B. Chemical damage to the liver.
                          C. Obstruction of common bile duct
                          D. Increased rate of hemolysis


                          Question 5

                          Convert the following temperature from Celsius to Fahrenheit
                          20 degrees C
                          Question options:

                          A. 39.2 degrees F
                          B. 14 degrees F
                          C. 68 degrees F
                          D. 77 degrees F


                          Solutions:

                          Question 1
                          Answer: E
                          Question 2
                          Answer: B
                          Question 3
                          Answer: A
                          Question 4
                          Answer: D
                          Question 5
                          Answer: C

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