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American Society of Microbiology ABMM Sample Questions:

1. A patient presents with a chronic, non-healing skin ulcer in a tropical region. Microscopic examination of a biopsy reveals amastigotes within macrophages. Culture of the biopsy on Novy-MacNeal-Nicolle (NNN) medium yields flagellated promastigotes. The MOST likely diagnosis is cutaneous leishmaniasis caused by:

A) Trypanosoma brucei gambiense
B) Trypanosoma cruzi
C) Leishmania tropica or Leishmania major
D) Leishmania donovani

2. A traveler returning from Southeast Asia presents with fever, headache, and a maculopapular rash that started on the trunk and spread to the extremities, including the palms and soles. Serological testing is positive for Orientia tsutsugamushi. The vector responsible for transmitting this organism is a:

A) Flea
B) Mosquito
C) Mite (chigger)
D) Tick

3. A laboratory worker sustains a needlestick injury while handling a blood sample known to be positive for Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg). The worker has completed the HBV vaccination series and has a documented anti-HBs titer >10 mIU/mL one month ago. What is the recommended post-exposure prophylaxis?

A) Hepatitis B immune globulin (HBIG) only
B) No treatment required
C) Both HBIG and HBV vaccine booster dose
D) Hepatitis B vaccine booster dose only

4. A clinical microbiology laboratory is implementing a new real-time PCR assay for the direct detection of Chlamydia trachomatis in urine samples. To ensure the accuracy of negative results, it is essential to include:

A) A high concentration of the Chlamydia trachomatis target sequence.
B) A melting curve analysis to confirm the specificity of the amplicon.
C) An internal amplification control to detect PCR inhibition.
D) A positive control containing Neisseria gonorrhoeae DNA.

5. A patient with a history of neutropenia following chemotherapy develops a severe pneumonia.
Bronchoalveolar lavage fluid reveals the presence of hyaline, septate hyphae with frequent dichotomous branching at acute angles. Culture yields a filamentous fungus that produces conidiophores with a vesicle bearing phialides that release chains of conidia. The MOST likely genus is:

A) Rhizopus
B) Aspergillus
C) Fusarium
D) Mucor

Solutions: